Column-Free Purification Methods for Recombinant Proteins Using Self-Cleaving Aggregating Tags
نویسندگان
چکیده
منابع مشابه
Streamlined protein expression and purification using cleavable self-aggregating tags
BACKGROUND Recombinant protein expression and purification remains a fundamental issue for biotechnology. Recently we found that two short self-assembling amphipathic peptides 18A (EWLKAFYEKVLEKLKELF) and ELK16 (LELELKLKLELELKLK) can induce the formation of active protein aggregates in Escherichia coli (E. coli), in which the target proteins retain high enzymatic activities. Here we further exp...
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ژورنال
عنوان ژورنال: Polymers
سال: 2018
ISSN: 2073-4360
DOI: 10.3390/polym10050468